MicroVue YKL-40 EIA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Quidel strongly recommends the use of an automated plate washer or wash bottle equipped with a pump and an 8-channel head to perform the wash steps in each assay. Use of a single or multichannel pipette as a wash device is strongly discouraged.

Protocol tips
Quidel strongly recommends the use of an automated plate washer or wash bottle equipped with a pump and an 8-channel head to perform the wash steps in each assay. Use of a single or multichannel pipette as a wash device is strongly discouraged.

Publication protocol

Five milliliter of fasting venous blood was drawn from each patient in the morning after being admitted for the measurement of biochemical indicators such as blood glucose and blood lipids. Another 5 ml of venous blood was drawn and centrifuged at 3000 r/min for 10 min. The supernatant serum layer was collected and then stored at −80°C. The levels of soluble ICAM-1, Lp-PLA2, and YKL-40 were measured by ELISA. For ICAM-1 and Lp-PLA2, the kit from R&D Systems Corporation (USA) was used, and for YKL-40, the kit from Quidel Corporation (USA) was used.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 using MicroVue YKL-40 EIA from Quidel.

Manufacturer protocol

Download the product protocol from Quidel for MicroVue YKL-40 EIA below.

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Videos

Check out videos that might be relevant for performing ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40 using MicroVue YKL-40 EIA from Quidel. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.