Human Cytochrome c Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Upstream tips
-Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Publication protocol

The cyt c was measured using a commercially available enzyme-linked immunosorbent assay (Quantikine; R&D System Inc, Minneapolis, MI). Troponin I, serum creatinine, LDL-C, uric acid, urea nitrogen, glucose, γ-GT, albumin, and hemoglobin were all tested on AU5800 autobiochemistry analyzer (Beckman Coulter, USA).

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Cytochrome c Quantikine ELISA Kit below.

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