Human Decorin DuoSet ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

Untreated, or control miRNA (QIAGEN), synthetic miR-181b, or DCN siRNA, was transfected into SF using HiPerFect according to manufacturer protocols. After 48 hours cell culture supernatant was collected and DCN measured using ELISA. Total RNA was harvested and RT-qPCR for DCN mRNA performed. Untreated, or control miRNA, or antagomiR-181b was transfected into DF using HiPerFect. After 48 hours supernatant was collected and DCN measured using ELISA.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Decorin DuoSet ELISA below.

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