Human Decorin ELISA Kit (DCN) (ab99998)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Discard the working standard dilutions after use – they do not store well.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Discard the working standard dilutions after use – they do not store well.

Publication protocol

EMOsis cc/TERT and CRL-4003 cells were seeded at a density of 1×106 cells per well in six-well plates and cultured in growth media until they reached 70–80% confluence. The cells were then starved for 16 h. The culture supernatants were collected after being incubated for 48 h with a vehicle (PBS) or 10, 100, or 500 nM of dienogest, and the level of decorin was assayed with 100 μl of cell-free culture supernatant using the Decorin Human ELISA kit (Abcam) according to the manufacturer's instructions. Absorbance was read at a wavelength of 450 nm using the Corona SH-1000 lab absorbance microplate reader (Corona Electric Co. Inc.). The sample concentrations were determined via interpolation based on the standard curve. The assay was performed three times, and the colony number was expressed as the mean±s.d.

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Manufacturer protocol

Download the product protocol from Abcam for Human Decorin ELISA Kit (DCN) (ab99998) below.

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