Human Dkk-1 DuoSet ELISA (DY1906)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

DuoSet Ancillary Reagent Kit and the corresponding Human Dkk-1 DuoSet ELISA were obtained from R&D Systems Inc. (Minneapolis, MN). Cell culture supernatants were diluted at a ratio 1:3 in 1% BSA in bovine serum and ELISA was performed according to the manufacturer’s protocol.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Dkk-1 DuoSet ELISA (DY1906) below.

Download manufacturer protocol

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