EGFR (Full-length) Human ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.	-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.	-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Upstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.
Protocol tips
-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.
Downstream tips
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Publication protocol

To supplement EGFR IHC, we performed an ELISA on 1–3 snap-frozen rectal biopsies which were pooled. Biopsies were incubated in a uticell extraction buffer (20-30ml; Invitrogen Cat # FNN0011) supplemented with 1 mM PMSF (Invitrogen) protease inhibitor cocktail (Sigma Chemicals) for 30–45 min at 4°C and then then centrifuged at 13,000 rpm for 10 min. The supernatant underwent protein estimation with Quant-iT assay kit (Invitrogen). For EGFR quantitation, 10 μg protein lysate was assessed with Invitrogen EGFR ELISA kit (KHR9061) according to the manufacturer’s directions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for EGFR (Full-length) Human ELISA Kit below.

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