Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.	-Wash by filling each well with Wash Buffer using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Upstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.
Protocol tips
-Wash by filling each well with Wash Buffer using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Publication protocol

Plasma levels of sCD163 and I-FABP were quantified by ELISA assay using Human sCD163 and I-FABP Duoset kits (R&D Systems) per the manufacturer’s instructions. Plasma samples were diluted 1:100 for sCD163 and 1:1,500 for I-FABP assays based on plasma titration studies to achieve levels within the range of the standard curve concentrations provided in the commercial ELISA kit according to the manufacturer’s recommendation. Each test was performed in duplicate with results reported as the average of duplicate results.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit below.

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