Human FABP2/I-FABP DuoSet ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

Levels of zonulin-1 (ALPCO, Salem, NH), intestinal fatty acid binding protein (I-FABP) (R&D Systems human FABP2 DuoSet ELISA system, Minneapolis, MN), and liposaccharide binding protein (LBP) (Hycult Biotech, Plymouth Meeting, PA) were measured by ELISAs.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human FABP2/I-FABP DuoSet ELISA below.

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