I-FABP, Human, ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. -Do not include sodium azide in buffers or wash solutions if an HRP-labeled antibody/conjugate will be used for detection.	-Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. -Use a humidified incubator to reduce drying effects in the outside wells (“edge effects”) or place a wet tissue or blotter paper under the plates in non-humidified incubators. -Do not stack plates.

Upstream tips
-Bring all reagents to room temperature before use. -Do not include sodium azide in buffers or wash solutions if an HRP-labeled antibody/conjugate will be used for detection.
Protocol tips
-Standards (duplicates or triplicates) and blank must be run with each plate to ensure accuracy. -Use a humidified incubator to reduce drying effects in the outside wells (“edge effects”) or place a wet tissue or blotter paper under the plates in non-humidified incubators. -Do not stack plates.

Publication protocol

The concentration of I-FABP was measured by Human I-FABP ELISA (Hycult Biotech, Uden, Netherlands), which is certified for both serum and urine. The assay was performed according to the manufacturer's instruction and the I-FABP concentration in serum is presented as ng/mL. To eliminate fluctuation in urine excretion, the urinary I-FABP was normalized to urinary creatinine and it is presented as pg/nmol of creatinine.

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Manufacturer protocol

Download the product protocol from Hycult Biotech for I-FABP, Human, ELISA kit below.

Download manufacturer protocol

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