Human Fibronectin ELISA Kit (ab108847)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Enzyme‐linked immunosorbent assay was carried out for fibronectin using the Abcam Human Fibronectin ELISA Kit (ab108847; Abcam Japan, Tokyo, Japan), according to the manufacturer's procedures. Briefly, cells were seeded onto each well at a concentration of 0.5 × 105 cells, and the supernatant was corrected at the indicated time with or without GO‐Y078.

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Manufacturer protocol

Download the product protocol from Abcam for Human Fibronectin ELISA Kit (ab108847) below.

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