Mouse GDNF ELISA Kit (ab171178)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum levels of total BDNF were measured by using six different ELISA kits, as listed in Table 2: human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF RapidTM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKineTM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex®/xMAP® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA). All samples were assayed in duplicate on each plate, in order to test also the intra-assay variation. A number of two plates per kit, of the same lot, were used over two different days, within the same week, in order to assess the inter-assay variation.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse GDNF ELISA Kit (ab171178) below.

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