Multi-Analyte ELISArray Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Briefly centrifuge any 1.5 ml tube in the kit before opening it in order to collect its contents at the bottom of the tube.	-Visually inspect the Wash Buffer Concentrate to ensure that all components are in solution. If any precipitates are visible, briefly shake the bottle to suspend any precipitate. -Avoid prolonged exposure of the Development Solution to light or contact with water, air, or extreme temperature.

Upstream tips
Briefly centrifuge any 1.5 ml tube in the kit before opening it in order to collect its contents at the bottom of the tube.
Protocol tips
-Visually inspect the Wash Buffer Concentrate to ensure that all components are in solution. If any precipitates are visible, briefly shake the bottle to suspend any precipitate. -Avoid prolonged exposure of the Development Solution to light or contact with water, air, or extreme temperature.

Publication protocol

The supernatants of the PBMC cultures, treated with rEhCRT, ConA, or without stimulus (RPMI-10% SFB), were tested for detection of interleukins IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17A, interferon gamma (IFNγ), tumor necrosis factor alpha (TNFα), granulocyte colony stimulating factor (GCSF), and transforming growth factor beta1 (TGFβ1) using a multianalytic ELISA array kit (MEH-003A, Qiagen), according to manufacturer's instructions. The concentrations of the cytokines are given in pg/ml and were calculated using the standard curve provided in the kit.

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Manufacturer protocol

Download the product protocol from Qiagen for Multi-Analyte ELISArray Kits below.

Download manufacturer protocol

Videos

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