HSP70 High Sensitivity ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.	-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Upstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.
Protocol tips
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Publication protocol

Ten-milliliter samples of morning urine were collected in dry plastic test tubes and centrifuged at room temperature for 15 min with rotational frequency of 3000 rpm. The urine supernatant was immediately frozen and stored in a refrigerator at the temperature of − 20 °C. We used the commercial enzyme-linked immunosorbent assay (ELISA) kits to determine the levels of HSPs 70 (ADI-EKS-715, Enzo Life Science, USA) and IL-10 (BMS 21512/BMS215/2TEN, eBiosciеnce, Austria).

For detection of anti-HSP70 antibodies, serum was obtained by centrifugation of the whole blood at room temperature for 20 min at 3000 rpm. Antibody levels were measured using commercial ELISA kits (ADI-EKS-750, Enzo Life Science, USA).

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for HSP70 High Sensitivity ELISA kit below.

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