Human HO 1 ELISA Kit (ab133064)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

In order to measure HMOX1 protein level in IT-901 treated Ly19 human DLBCL, Ly19 cells were lysed with extraction reagent included in the HO-1 Human ELISA Kit (abcam). Analysis was performed per manufacturer’s instructions. Optical density (OD) was determined, using a microplate autoreader (Bio-Tek Optiplex 755 Microplate Reader) at 450 nm. HMOX1 protein concentration of the samples was determined based on a specific HMOX1 standard curve.

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Manufacturer protocol

Download the product protocol from Abcam for Human HO 1 ELISA Kit (ab133064) below.

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