Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use.	-When mixing or reconstituting protein solutions, always avoid foaming. -To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. -Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution.

Upstream tips
-Bring all reagents to room temperature before use.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. -Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution.

Publication protocol

sICAM-1 concentration was measured using a Quantikine® Human ICAM-1/CD54 Allele-specific Immunoassay kit (Catalog number: DCD540) from R&D Systems Europe Ltd., Abingdon, England, in compliance with the manufacturer’s instructions. The detection range for the kit is between 0.00–50 ng/mL CSF and serum were diluted 20-fold prior to analysis.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit below.

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