Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature before use. |
-When mixing or reconstituting protein solutions, always avoid foaming.
-To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
-Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. |
|
Upstream tips |
-Bring all reagents to room temperature before use. |
Protocol tips |
-When mixing or reconstituting protein solutions, always avoid foaming.
-To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
-Substrate Solution should remain colorless until added to the plate. Keep Substrate Solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution. |
Publication protocol
sICAM-1 concentration was measured using a Quantikine® Human ICAM-1/CD54 Allele-specific Immunoassay kit (Catalog number: DCD540) from R&D Systems Europe Ltd., Abingdon, England, in compliance with the manufacturer’s instructions. The detection range for the kit is between 0.00–50 ng/mL CSF and serum were diluted 20-fold prior to analysis.
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Manufacturer protocol
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