Human IGF1 ELISA Kit (ab100545)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Prepare serially diluted standards immediately prior to use. Always prepare a fresh set of standards for every use. -Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Do not vortex the standard during reconstitution, as this will destabilize the protein. -Keep the standard dilutions on ice while during preparation, but the ELISA procedure should be done at room temperature.	-Discard working standard dilutions after use as they do not store well.

Upstream tips
-Prepare serially diluted standards immediately prior to use. Always prepare a fresh set of standards for every use. -Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Do not vortex the standard during reconstitution, as this will destabilize the protein. -Keep the standard dilutions on ice while during preparation, but the ELISA procedure should be done at room temperature.
Downstream tips
-Discard working standard dilutions after use as they do not store well.

Publication protocol

Plasma blood glucose, serum insulin and a full blood lipid profile were assessed through the chemical PathCare laboratories (Stellenbosch) to assist in classify women as having the MetS or not. Female serum testosterone, sex hormone binding globulin (SHBG) and free androgen index (FAI) were also assessed. Highly sensitive enzyme linked immunosorbent assay (ELISA) kits were used to quantify CRP (CRP human simple set ELISA kit®, Abcam, UK) [20], and IGF-1 concentration (IGF-1 human ELISA kit®, Abcam, UK) [21]. Serum samples were diluted 25,000 times for CRP, and 20 times for IGF-1. The optical densities of all participant samples were measured using an EL800 universal microplate reader (Bio-tek Instruments, South Africa) at 450 nm, within 15 min after the stop solution was added.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Human - IGF-I using Human IGF1 ELISA Kit (ab100545) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Human IGF1 ELISA Kit (ab100545) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Human - IGF-I using Human IGF1 ELISA Kit (ab100545) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.