KIM-1 (human) ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C.	-Include a standard curve each time the assay is performed. -Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents. -In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay. -Consistent, thorough washing of each well is critical.

Upstream tips
-All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C.
Protocol tips
-Include a standard curve each time the assay is performed. -Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents. -In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay. -Consistent, thorough washing of each well is critical.

Publication protocol

Urine samples (10 ml) were collected and stored frozen (-20°C) until assessment of biomarker concentrations took place. Urinary concentrations of NGAL and KIM-1 were assessed by ELISA (BPD-KIT-036 from BioPorto Diagnostics and ADI-900-226-0001 from Enzo Life Science, respectively).

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for KIM-1 (human) ELISA kit below.

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