Human LBP DuoSet ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

Peripheral blood, drawn from each subject on the morning after PSG, was centrifuged at 3000 rpm for 15 min, and serum was stored at − 70 °C for analysis. Concentrations of serum LBP, IL-1β, IL-6, TNF-ɑ were measured using commercially available ELISA kits (R&D Systems, Minneapolis, MN, USA) in duplicate according to the manufacturer’s instruction. An automatic biochemical analyzer (UniCel DxC 800 Synchron, Beckman Coulter, Inc., Brea, CA, USA) was used to test the serum level of lipids.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human LBP DuoSet ELISA below.

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