RNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.		- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

RNA was isolated from FACS-sorted HSCs with the RNeasy Micro kit (Qiagen). Paired end libraries were generated with Illumina TruSeq RNA kit. Alignment was performed by RUM (Grant et al., 2011). Cufflinks and Cuffdiff (Trapnell et al., 2010) were used for transcript reconstruction, quantification, and differential expression analysis.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

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