RNeasy Micro Kit

RNA isolation / purification Cells - primary hematopoietic stem cells

Experiment
RNA isolation / purification Cells - primary hematopoietic stem cells
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

RNA was isolated from FACS-sorted HSCs with the RNeasy Micro kit (Qiagen). Paired end libraries were generated with Illumina TruSeq RNA kit. Alignment was performed by RUM (Grant et al., 2011). Cufflinks and Cuffdiff (Trapnell et al., 2010) were used for transcript reconstruction, quantification, and differential expression analysis.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary hematopoietic stem cells using RNeasy Micro Kit from Qiagen.

Paper title
Long Non-coding RNAs Control Hematopoietic Stem Cell Function
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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