Human Leptin ELISA Kit (ab100581)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The blood specimens were obtained from African American patients on the day of colonoscopy. Blood samples were centrifuged for 15 min at a speed of 3,500 g at 4°C then sera were separated and stored at −80°C. Serum concentrations of adiponectin (pg/ml), leptin (pg/ml), TNF-α (pg/ml), and IGF-1 (ng/ml), were quantified using commercially available ultrasensitive ELISA kits according to the manufacturer’s recommendations (Abcam, Cambridge, MA, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Human Leptin ELISA Kit (ab100581) below.

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