Human Leptin PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Venous blood samples were centrifugated at 3000 g at 4 °C for 15 min. Serums were frozen at -20 °C until enzyme-linked immunosorbent assay (ELISA) analysis. Leptin serum concentration was measured by using human leptin ELISA kit (Catalog No. EK0437,Boster Biological Technology Co., Ltd.). The samples were read on a Synergy HT, Multi-Detection Microplate Reader, BIO-TEK plate reader by measuring the absorbance at a wavelength of 450 nm. The ELISAs were performed according to the instructions of the manufacturerand the intra and interassay coefficients of variation were < 10%. All assays were conducted twice in the same occasion and the average value obtained, and conducted within the same laboratory under the same conditions.

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Manufacturer protocol

Download the product protocol from BosterBio for Human Leptin PicoKine™ ELISA Kit below.

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