MMP9 Human ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Buffer Concentrates should be brought to room temperature and should be diluted before starting the test procedure. -If crystals have formed in the Buffer Concentrates, warm them gently until they have completely dissolved.	-The standard has to be used immediately after reconstitution and cannot be stored. -The Biotin-Conjugate should be used within 30 minutes after dilution. - Do not allow wells to dry after washing steps.

Upstream tips
-Buffer Concentrates should be brought to room temperature and should be diluted before starting the test procedure. -If crystals have formed in the Buffer Concentrates, warm them gently until they have completely dissolved.
Protocol tips
-The standard has to be used immediately after reconstitution and cannot be stored. -The Biotin-Conjugate should be used within 30 minutes after dilution. - Do not allow wells to dry after washing steps.

Publication protocol

ELISA test was performed to detect MMP-9 levels in A375 and A2058 culture supernatants using MMP-9 Human ELISA Kit (cat. n. BMS2016-2 - Invitrogen Corporation, Carlsbad, CA, United States) according to the manufacturer’s instructions. Whereas, Human MMP-9 Quantikine ELISA KIT (cat. n. DMP900 - R&D Systems, Minneapolis, MN, United States) was used to detect serum levels of MMP-9 in melanoma patients enrolled in this study. Optical density (OD) was measured by Tecan ELISA plate reader (Tecan, Männedorf, Switzerland). Linear regression analysis was performed to generate standard curve from average absorbance of standards. MMP-9 concentrations were calculated fitting the average of duplicate ODs of each sample with standard curve.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MMP9 Human ELISA Kit below.

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