Human MPO/Myeloperoxidase PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Nitric oxide (NO) was assessed using the Griess reagent based on methods previously described (Green et al. 1982). Hydrogen peroxide (H2O2) was measured using a colorimetric kit (Bio-diagnostic, Egypt) as previously described (Fossati et al. 1980). Catalase was measured using a colorimetric kit (Bio-diagnostic, Egypt) according to Aebi (1984). Human myeloperoxidase (MPO), Tumor necrosis factor-α (TNF-α) and Neutrophil elastase were measured using the ELISA kits MPO (Boster Immunoleader, USA), Human TNF-α (Boster Immunoleader, USA) and elastase (ASSAY PRO, USA), respectively, according to the manufacturer’s instructions. Non-activated neutrophils were used as negative controls.

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Manufacturer protocol

Download the product protocol from BosterBio for Human MPO/Myeloperoxidase PicoKine™ ELISA Kit below.

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