Human MCP-1 ELISA Kit (ab179886)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Quantification of several inflammatory cytokines [IL1-β (ab46052), IL-4 (ab100570), IL-6 (ab46027), IL-6R (ab46029), IL-8 (ab46032), IL-10 (ab46034), IL-13 (ab100553)], cell adhesion markers [ICAM-1 (ab174445), CCL-2 (MCP-1, ab179886)], chemokines [CCL-4 (MIP-1β, ab100597), CCL-5 (RANTES, ab174446), CXCL-10 (IP-10, ab83700), TIMP-2 (ab100653)], and other known CVD marker [CD-40 (ab99990) and HSP-70 (ab187399)] were performed and normalized for 1 µg total protein of cell lysates and EV lysates using Human ELISA Kits (Abcam Ltd., Cambridge, UK), according to their manufacturer’s instructions. Cell-free medium-derived EV (cEV) served as a negative control. Optical density of standards and samples were measured using a Multiskan™ FC Microplate Absorbance Reader (Thermo Scientific, Belgium).

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Manufacturer protocol

Download the product protocol from Abcam for Human MCP-1 ELISA Kit (ab179886) below.

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