Human MCP-1 / CCL2 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Enzyme-linked immunosorbent assay (ELISA) kits for superoxide dismutase (SOD), monocyte chemotactic protein 1 (MCP-1), vascular cell adhesion molecule 1 (VCAM-1), and human angiotensin-converting enzyme (ACE) were purchased from Boster Biotechnology Inc. (Wuhan, China).

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Manufacturer protocol

Download the product protocol from BosterBio for Human MCP-1 / CCL2 PicoKine™ ELISA Kit below.

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