Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
-Equilibrate all reagents to room temperature (18-25°C) prior to use. |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
-Unused well strips should be returned to the plate packet and
stored at 4°C.
-Don’t store the diluted HRP-Streptavidin solution for next day use. |
Upstream tips |
-Equilibrate all reagents to room temperature (18-25°C) prior to use. |
Protocol tips |
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background. |
Downstream tips |
-Unused well strips should be returned to the plate packet and
stored at 4°C.
-Don’t store the diluted HRP-Streptavidin solution for next day use. |
Publication protocol
Blood samples were collected immediately after AKI diagnosis. Acquired blood samples were rested for 30 min and subsequently centrifuged at 3000 rpm at 4 °C for 10 min, and supernatant plasma was stored and frozen at − 80 °C. Endostatin, NGAL and cystatin C in plasma were measured with a commercially available enzyme-linked immunosorbent assay (ELISA) kit (ab100508, Lot GR3183088-1 (endostatin); ab119600, Lot GR316206-1 (NGAL); ab179883, Lot GR308840–1 (cystatin C); Abcam, UK). The biomarkers were measured by technicians who were blind to clinical data and the physicians in charge were blind to the biomarker test results.
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Manufacturer protocol
Download the product protocol from Abcam for Human Lipocalin-2 ELISA Kit (NGAL) (ab119600) below.
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