Human Lipocalin-2 ELISA Kit (NGAL) (ab119600)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C. -Don’t store the diluted HRP-Streptavidin solution for next day use.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C. -Don’t store the diluted HRP-Streptavidin solution for next day use.

Publication protocol

Blood samples were collected immediately after AKI diagnosis. Acquired blood samples were rested for 30 min and subsequently centrifuged at 3000 rpm at 4 °C for 10 min, and supernatant plasma was stored and frozen at − 80 °C. Endostatin, NGAL and cystatin C in plasma were measured with a commercially available enzyme-linked immunosorbent assay (ELISA) kit (ab100508, Lot GR3183088-1 (endostatin); ab119600, Lot GR316206-1 (NGAL); ab179883, Lot GR308840–1 (cystatin C); Abcam, UK). The biomarkers were measured by technicians who were blind to clinical data and the physicians in charge were blind to the biomarker test results.

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Manufacturer protocol

Download the product protocol from Abcam for Human Lipocalin-2 ELISA Kit (NGAL) (ab119600) below.

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