Quick-RNA™ MiniPrep Plus

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	In order to monitor the response of Dex the transfected cells, we also monitored AQP5 and GAPDH expression at the mRNA level: total RNA was extracted from a 100 μL trypsinized EpH4 cell suspension with the Quick-RNA MiniPrep Kit (Zymo Research). All steps were performed according to manufacturer's instructions. Expression changes were monitored as described above (Extraction of mRNA and analysis with qPCR), except that the SensiMix SYBR No-ROX One-step Kit (Bioline) was used for onestep qPCR.

Protocol tips

In order to monitor the response of Dex the transfected cells, we also monitored AQP5 and GAPDH expression at the mRNA level: total RNA was extracted from a 100 μL trypsinized EpH4 cell suspension with the Quick-RNA MiniPrep Kit (Zymo Research). All steps were performed according to manufacturer's instructions. Expression changes were monitored as described above (Extraction of mRNA and analysis with qPCR), except that the SensiMix SYBR No-ROX One-step Kit (Bioline) was used for onestep qPCR.

Publication protocol

In order to monitor the response of Dex the transfected cells, we also monitored AQP5 and GAPDH expression at the mRNA level: total RNA was extracted from a 100 μL trypsinized EpH4 cell suspension with the Quick-RNA MiniPrep Kit (Zymo Research). All steps were performed according to manufacturer's instructions. Expression changes were monitored as described above (Extraction of mRNA and analysis with qPCR), except that the SensiMix SYBR No-ROX One-step Kit (Bioline) was used for onestep qPCR.

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Manufacturer protocol

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