Quick-RNA™ MiniPrep Plus

RNA isolation / purification Cells - primary human epithelial cells

Experiment
RNA isolation / purification Cells - primary human epithelial cells
Product
Quick-RNA™ MiniPrep Plus from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Protocol tips
In order to monitor the response of Dex the transfected cells, we also monitored AQP5 and GAPDH expression at the mRNA level: total RNA was extracted from a 100 μL trypsinized EpH4 cell suspension with the Quick-RNA MiniPrep Kit (Zymo Research). All steps were performed according to manufacturer's instructions. Expression changes were monitored as described above (Extraction of mRNA and analysis with qPCR), except that the SensiMix SYBR No-ROX One-step Kit (Bioline) was used for onestep qPCR.

Publication protocol

In order to monitor the response of Dex the transfected cells, we also monitored AQP5 and GAPDH expression at the mRNA level: total RNA was extracted from a 100 μL trypsinized EpH4 cell suspension with the Quick-RNA MiniPrep Kit (Zymo Research). All steps were performed according to manufacturer's instructions. Expression changes were monitored as described above (Extraction of mRNA and analysis with qPCR), except that the SensiMix SYBR No-ROX One-step Kit (Bioline) was used for onestep qPCR.

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human epithelial cells using Quick-RNA™ MiniPrep Plus from Zymo Research.

Paper title
Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation
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Manufacturer protocol

Download the product protocol from Zymo Research for Quick-RNA™ MiniPrep Plus below.

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Videos

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