Human PDGF BB ELISA Kit (ab100624)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Ready-to-use Sandwich ELISAs for human platelet-derived growth factor (PDGF; Abcam, Cambridge, UK), human prostaglandin E2 (PGE2; Abcam), and human vascular endothelial growth factor (VEGF, Abcam) were used to quantify the growth factors. After seven days of treatment supernatants were used undiluted or diluted as required from 1:1 to 1:10 fold and proceeded according to the manufacturer’s instruction. All measurements were performed in duplicates at 450 nm with a Spectrostar microplate reader (BMC Labtech, Ortenberg, Germany).

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Manufacturer protocol

Download the product protocol from Abcam for Human PDGF BB ELISA Kit (ab100624) below.

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