Human Prolactin DuoSet ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

The concentration of IGFBP1 and PRL in DSC CM was measured using Human Duo-Set enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems), according to the manufacturer’s instructions. Where the analyte reading was below the sensitivity of the assay, an arbitrary value of half of the lowest standard for that experiment was used. All R&D Systems Duo-Set ELISAs typically have coefficient of variation (CV) values less than 10% across the standard curve for both intra- and inter-assay precision.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Prolactin DuoSet ELISA below.

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