RNeasy Mini Kit

RNA isolation / purification Cells - primary human keratinocytes

4/5 - 1 Review - 100% recommends this

Experiment

RNA isolation / purification Cells - primary human keratinocytes

Product

RNeasy Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Total RNA was purified using RNeasy Mini Kits (Qiagen Inc., Valencia, CA). RNA samples were treated with DNase I (Qiagen, Inc.) prior to synthesis of cDNA using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen/Thermo Fisher Scientific).

Protocol tips
Total RNA was purified using RNeasy Mini Kits (Qiagen Inc., Valencia, CA). RNA samples were treated with DNase I (Qiagen, Inc.) prior to synthesis of cDNA using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen/Thermo Fisher Scientific).

Publication protocol

Total RNA was purified using RNeasy Mini Kits (Qiagen Inc., Valencia, CA). RNA samples were treated with DNase I (Qiagen, Inc.) prior to synthesis of cDNA using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen/Thermo Fisher Scientific).

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Reviews

Scientist

Review date

2018-09-21

Protocol

Standard

Protocol tips

Add dnase1

Product rating

Would recommend?

Yes

What worked well?

Easy
Fast

What could be improved?

Dna contamination

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Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human keratinocytes using RNeasy Mini Kit from Qiagen.

Paper title

Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

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