Human Prolactin/PRL ELISA Kit

ELISA Human - PRL

Experiment
ELISA Human - PRL
Product
Human Prolactin/PRL ELISA Kit from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and
stored at 4°C.

Publication protocol

Secreted levels of prolactin, IGFBP-1,Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-1 beta (IL-1β), Granulocyte-macrophage colony-stimulating factor (GM-CSF), RANTES (also named as C-C motif Chemokine ligand 5 (CCL5)) and monocyte chemoattractant protein 1 (MCP1) into cell culture supernatant were determined using matching ELISA kits for prolactin (ab226901, Abcam), IGFBP-1 (ab100539, Abcam), IL-6 (ab46042, Abcam), IL-8 (ab46032, Abcam), IL-1β (ab214025, Abcam), GM-CSF (ab100529, Abcam) RANTES (ab100633, Abcam) and MCP-1 (ab100586, Abcam) according to the manufacturer’s instructions. The detection zone of each ELISA kit was listed as follows: 39.06 pg/ml - 2500 pg/ml for prolactin, 2.74 pg/ml -2000 pg/ml for IGFBP-1, 1.56 pg/ml - 50 pg/ml for IL-6, 62.5 pg/ml - 2000 pg/ml for IL-8, 14.06 pg/ml - 900 pg/ml for IL-1β, 2.05 pg/ml - 500 pg/ml for GM-CSF, 2.74 pg/ml - 2000 pg/ml for RANTES, 2 pg/ml - 500 pg/ml for MCP-1.

Full paper   Login or join for free to view the full paper.

Reviews

Human Prolactin/PRL ELISA Kit from Abcam has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Human - PRL using Human Prolactin/PRL ELISA Kit from Abcam.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abcam for Human Prolactin/PRL ELISA Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Human - PRL using Human Prolactin/PRL ELISA Kit from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms