Human Prolactin/PRL ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Secreted levels of prolactin, IGFBP-1,Interleukin-6 (IL-6), Interleukin-8 (IL-8), Interleukin-1 beta (IL-1β), Granulocyte-macrophage colony-stimulating factor (GM-CSF), RANTES (also named as C-C motif Chemokine ligand 5 (CCL5)) and monocyte chemoattractant protein 1 (MCP1) into cell culture supernatant were determined using matching ELISA kits for prolactin (ab226901, Abcam), IGFBP-1 (ab100539, Abcam), IL-6 (ab46042, Abcam), IL-8 (ab46032, Abcam), IL-1β (ab214025, Abcam), GM-CSF (ab100529, Abcam) RANTES (ab100633, Abcam) and MCP-1 (ab100586, Abcam) according to the manufacturer’s instructions. The detection zone of each ELISA kit was listed as follows: 39.06 pg/ml - 2500 pg/ml for prolactin, 2.74 pg/ml -2000 pg/ml for IGFBP-1, 1.56 pg/ml - 50 pg/ml for IL-6, 62.5 pg/ml - 2000 pg/ml for IL-8, 14.06 pg/ml - 900 pg/ml for IL-1β, 2.05 pg/ml - 500 pg/ml for GM-CSF, 2.74 pg/ml - 2000 pg/ml for RANTES, 2 pg/ml - 500 pg/ml for MCP-1.

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Manufacturer protocol

Download the product protocol from Abcam for Human Prolactin/PRL ELISA Kit below.

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