Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Following manufacturer instructions, enzyme-linked immunosorbent assay was used to measure plasma C-reactive protein (CRP) and adiponectin levels (eBioscience) and retinol-binding protein 4 (RBP4) levels (Abcam, Cambridge, England).

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Manufacturer protocol

Download the product protocol from Abcam for Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897) below.

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