Human SDC1 / Syndecan-1 ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.	-Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Upstream tips
-Bring all reagents and samples to room temperature (18–25 °C) before use.
Protocol tips
-Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. -Read at 450 nm immediately after adding STOP solution.

Publication protocol

Mice were obtained from the animal center of NCKU. Before the injection of PBS or recombinant NS1, blood from 8- to 12-week-old BALB/c mice was collected by orbital sinus sampling with 10% citrate. Next, the mice were intravenously injected with 50 μg of NS1 or 100 μl of PBS. After the intravenous injection, blood from the mice was immediately collected by orbital sinus sampling and every 24 h thereafter until 120 h after the injection. The plasma concentrations of NS1, MIF, HPA-1, and MMP were analyzed by ELISA. For the peritoneal challenge, 500 μl of PBS, 50 μg of NS1, 50 μg of E or 50 μg of prM was injected intraperitoneally. After 24 h, the mice were sacrificed, and the abdominal cavities were washed with 5 ml of PBS. The resultant peritoneal lavage was collected, and the concentrations of MIF, HPA-1 and CD138 were quantified by ELISA.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Human SDC1 / Syndecan-1 ELISA Kit below.

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