Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

The cytopsins were further subjected to Wright-Giemsa staining and immunofluorescent staining for Club cell 10-kDa protein (CC10, sc365992, Santa Cruz Biotechnology, Inc., Santa Cruz, CA), and SHH (06-1106, Millipore, Billerica, MA), whereas the supernatants were stored at −80 °C until further ELISA assays for N-SHH (DSHH00, R&D systems, Inc., Minneapolis, MN) and Bradford assays for protein quantification (Beyotime, Shanghai, China) were undertaken.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit below.

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