Human TNF-alpha Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

The concentrations of studied parameters in serum samples were measured by the enzyme-linked immunosorbent assay (ELISA) using commercially available kits according to manufacturer’s detailed instructions: for Tumor Necrosis Factor alpha (TNF-α)—Quantikine ELISA Human TNF-α kit, for Fas—Quantikine ELISA Human Fas/TNFRSF6 kit, for Fas Ligand (FasL)—Quantikine ELISA Human Fas Ligand/TNFSF6 kit, for soluble TNF receptor I (sTNF RI)—Quantikine ELISA Human sTNF RI/TNFRSF1A kit and for soluble TNF receptor II (sTNF RII)—Quantikine ELISA Human TNF RII/TNFRSF1B kit (all kits from R&D Systems, MN, USA). The coefficients of variance for intra-assay and inter-assay were below 8% and 10%, respectively.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human TNF-alpha Quantikine ELISA Kit below.

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