Human TNF Alpha PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The standard solution in IL-6, −10 and TNF-α ELISA kits was diluted to 800, 600, 400, 300, 200 and 100 pg/ml for determination of standard curve. The biotin-labeled antibodies were diluted 100 times to prepare it into the working solution. After 100 µl sample or standard substance was added into each well, the plate was sealed for reaction at 37°C for 60 min. After the reaction was over, IL-6, −10 and TNF-α antibodies were added following strictly the instructions of the kit for reaction for 90 min. After the reaction was over, 300 µl buffer was added. After standing for 1 min, the plate was patted dry and washed 5 times. Then 100 µl avidin-peroxidase compound was added, followed by plate sealing and reaction at 37°C for 60 min. The OD value at the wavelength of 450 nm of each well was measured after the stop buffer was added. Finally, the sample concentration of each group was analyzed and calculated using CurveExpert 1.5 software.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Human - TNF-alpha using Human TNF Alpha PicoKine™ ELISA Kit from BosterBio.

Manufacturer protocol

Download the product protocol from BosterBio for Human TNF Alpha PicoKine™ ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Human - TNF-alpha using Human TNF Alpha PicoKine™ ELISA Kit from BosterBio. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.