Human TGF beta 1 ELISA Kit (ab100647)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Quantification of EMT-marker proteins in representative human blood serum samples of patients with PDAC (n = 16), chronic pancreatitis (n = 8) and age-matched healthy controls (n = 8) was conducted with ELISA for soluble E-cadherin (ready-to-use kit 99-1700, 1:6.67, Invitrogen, Carlsbad, CA, USA), fibronectin (ready-to-use kit EHFN1, 1:10,000, ThermoScientific, Waltham, MA, USA) and TGF beta 1 (ready-to-use kit ab100647, abcam, Cambridge, UK) as written in the fabricants’ manuals.

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Manufacturer protocol

Download the product protocol from Abcam for Human TGF beta 1 ELISA Kit (ab100647) below.

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