Human VEGF PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

All participants were fasted overnight, and approximately 3 mL of elbow venous blood was collected from each participant in the morning and stored in ice. Urinary albumin (UALB) and urine creatinine (Cr) of all participants were measured for 24 h, with their ratio (UALB/Cr) being calculated. The elbow venous blood was centrifuged for 10 min at 1,200 g at 4°C. Supernatant was separated and packed, and then immediately stored at −80°C for further detection. Enzyme‐linked immunosorbent assay (Wuhan Boster Biological Engineering Co., Ltd., Wuhan, China) was used to detect the concentrations of IGF‐1, TNF‐α and VEGF165 in serum. Absorbance (A) of each well [optical density (OD)] value was measured by full wavelength spectrophotometer, with a detection wavelength of 450 nm and calibration wavelength of 570 nm. A standard curve was drawn using the A (OD) value as the ordinate, and standard substance concentration as abscissa to calculate concentrations of IGF‐1, TNF‐α and VEGF165 in serum.

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Manufacturer protocol

Download the product protocol from BosterBio for Human VEGF PicoKine™ ELISA Kit below.

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