Human Von Willebrand Factor ELISA Kit (VWF) (ab108918)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

We measured the levels of seven serum complements, ten plasma coagulations and three serum inflammatory cytokines by commercially available ELISA kits. The serum complements included C3a (Catalog No. 550499, BD), C4a (Catalog No. 550947, BD), C5a (Catalog No. 557965, BD), mannose-binding lectin-associated serine proteinase 2 (MASP2) (Catalog No. HK326, Hycult Biotech), C1q (Catalog No. ab170246, Abcam), C7 (Catalog No. ab125964, Abcam) and factor I (FI) (Catalog No. ab195460, Abcam). The plasma coagulations included factor 7 (F7) (Catalog No. ab108829, Abcam), factor 9 (F9) (Catalog No. ab108831, Abcam), factor 12 (F12) (Catalog No. ab108835, Abcam), factor 13 (F13) (Catalog No. ab108836, Abcam), fibrinogen (FIB) (Catalog No. ab108842, Abcam), thrombin-antithrombin complex (TAT) (Catalog No. ab108907, Abcam), Von Willebrand factor (VWF) (Catalog No. ab108918, Abcam), protein S (PROS) (Catalog No. ab125969, Abcam), D-dimer (Catalog No. ab196269, Abcam) and antithrombin-III (ATIII). (Catalog No. DSPC10, RD). The serum inflammatory cytokines included tumor necrosis factor-receptor II (TNF-RII) (Catalog No. DRT200, RD), interleukin (IL)-6 (Catalog No. 430507, BioLegend) and IL-8 (Catalog No. D8000C, RD). In addition, the level of serum C4 was measured in the clinical laboratory using nephelometric method.

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Manufacturer protocol

Download the product protocol from Abcam for Human Von Willebrand Factor ELISA Kit (VWF) (ab108918) below.

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