Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Blood samples were collected in three groups of rats at day 42 after WJ-MSC-apelin infusion, and serum and EDTA plasma were stored at − 20 °C. Concentrations of Apelin, C-peptide, and insulin in rat plasma were measured after WJ-MSC-apelin infusion. Plasma apelin concentrations of three groups of rats were quantified with an ELISA kit (Phoenix Pharmaceuticals, Belmont, CA, USA) following the manufacturer’s instructions. The concentrations of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and adiponectin (APN) were determined with ELISA kits (R&D Systems, Minneapolis, MN, USA). All samples were analyzed with duplicates and samples with coefficient of variation (CV) values > 15% were excluded. Blood biochemical analyses and laboratory assays including hematologic, tumor, and immune indexes were conducted by routine clinical lab methods at 6 weeks after treatments.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Rat - Adiponectin using Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit from R&D Systems.

Manufacturer protocol

Download the product protocol from R&D Systems for Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Rat - Adiponectin using Rat Total Adiponectin/Acrp30 Quantikine ELISA Kit from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.