Rat Activin-A ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

Analysis of unconcentrated conditioned medium cytokines was performed using the RayBio® Biotin Label-based Rat Antibody Array 1 (RayBiotech, Norcross, GA, USA) according to the manufacturer's instructions. To verify the results, the same unconcentrated conditioned medium was assayed by ELISA for bFGF, VEGF, TGF-β1, IGF-1, HGF and IL-10. The blood samples were assayed by ELISA for IL-1β, IL-6, TNF-α and IL-10 whereas intestinal tissue samples were assayed by ELISA for IL-1β, IL-6, TNF-α, TGF-β1, Activin A and IL-10.

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Manufacturer protocol

Download the product protocol from Raybiotech for Rat Activin-A ELISA below.

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