Rat BDNF PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

BDNF concentration was measured by ELISA kit from Boster Biological Technology Co., California, USA. A monoclonal antibody from mouse specific for BDNF and the assay Avidin-Biotin-Peroxidase complex was used to bind the detection antibody. HRP substrate 3,3′,5,5′-tetramethylbenzidine (TMB) was used to visualize HRP enzymatic reaction which produces a blue colour product that changed into yellow after adding acidic stop solution. The density of yellow colour, measured at 450 nm, is proportionate to the BDNF concentration in the sample.

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Manufacturer protocol

Download the product protocol from BosterBio for Rat BDNF PicoKine™ ELISA Kit below.

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