Rat C-Reactive Protein/CRP DuoSet ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.	-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Upstream tips
-Bring all reagents to room temperature before use. Allow all components to sit for a minimum of 15 minutes with gentle agitation after initial reconstitution. -Working dilutions should be prepared and used immediately.
Protocol tips
-A standard curve should be generated for each set of samples assayed. -It is suggested to start Reagent Diluent optimization for serum and plasma samples by using PBS supplemented with 10-50% animal serum. -Do not use buffers with animal serum to reconstitute or dilute the Detection Antibody or Streptavidin-HRP B.

Publication protocol

Body weight was measured every four days. FBG levels of rats were determined by a glucose-meter (ONETOUCH, Ultra, Lifescan, USA). LDL-C, HDL-C, TC and TG concentrations in serum were detected using commercially available kits from Nanjing Jiancheng Bioengineering Institute (China). TNF-α, IL-6, CRP, Resistin and FINS levels were evaluated by commercial rat enzyme-linked immune sorbent assay (ELISA) kits (R & D Systems China Co. Ltd., Shanghai, China). Insulin resistance containing insulin resistance index (HOMA-IR) and insulin sensitivity index (HOMA-ISI) were calculated using the following formulas: HOMA-ISI = −Ln (FBG × FINS), HOMA-IR = FINS × FBG/22.5.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Rat - C-Reactive Protein/CRP using Rat C-Reactive Protein/CRP DuoSet ELISA from R&D Systems.

Manufacturer protocol

Download the product protocol from R&D Systems for Rat C-Reactive Protein/CRP DuoSet ELISA below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Rat - C-Reactive Protein/CRP using Rat C-Reactive Protein/CRP DuoSet ELISA from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.