Rat/Mouse Cytochrome c Quantikine ELISA Kit

ELISA Rat - Cytochrome c

Experiment
ELISA Rat - Cytochrome c
Product
Rat/Mouse Cytochrome c Quantikine ELISA Kit from R&D Systems
Manufacturer
R&D Systems

Protocol tips

Upstream tips
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.

Publication protocol

A homogenate portion was centrifuged at 10,000 rpm for 30 min at 4 °C. Cytosolic fraction (the supernatant) was used to measure cytochrome C by an ELISA kit (R&D Systems, Minneapolis, MN).

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Papers

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Manufacturer protocol

Download the product protocol from R&D Systems for Rat/Mouse Cytochrome c Quantikine ELISA Kit below.

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