Rat Fractalkine ELISA Kit (CX3CL1) (ab100760)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Blood samples from the ethanol experiments in rats were immediately collected and centrifuged (2,000 × g for 15 min) and plasmas were kept at −80°C for immunoassay analyses. CXCL12 (SDF-1), CX3CL1 (fractalkine), and CCL11 (eotaxin-1) were measured in rat plasma with commercial enzyme-linked immunosorbent assay (ELISA) kits in 96-well plate format following the manufacturer’s instructions: ELISA Kit for SDF-1 (product #: SEA122Ra; Cloud-Clone Corp., Wuhan, Hubei, PR China), Rat Fractalkine ELISA Kit (CX3CL1) (product #: ab100760; Abcam, Cambridge, UK), and Rat Eotaxin 1 (Eotaxin 1/CCL11/ECF) ELISA Kit (product #: CSB-E07319r; Cusabio, College Park, MD, USA). The plates were read at 450 nm within 30 min at a set wavelength of 540 nm or 570 nm. Data are expressed as nanograms of protein per milliliter of plasma.

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Manufacturer protocol

Download the product protocol from Abcam for Rat Fractalkine ELISA Kit (CX3CL1) (ab100760) below.

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