Rat sE-Selectin ELISA Kit (sCD62E) (ab171334)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The homogenate/serum levels of the following parameters were evaluated using the corresponding ELISA kits as shown in parentheses: IL-1β, sE-selectin (Abcam Inc., Cambridge, UK, Cat. No. ab100768 and ab171334, respectively), iNOS, eNOS (EIAab, Wuhan, China, Cat. No. E0837r and E0868r, respectively), B cell leukemia/lymphoma-2 (Bcl-2; USCN Life Science Inc., Wuhan, China, Cat. No. E90778Bo) and caspase-3 (Cusabio Biotech Co., Wuhan, China, Cat. No. CSB-E08857r). Each biomarker was processed according to the manufacturers' procedures provided. Serum total antioxidant capacity (TAC) was measured using the method of Benzie and Starin [19]. Briefly, ferric tripyridyltriazine (Fe3+-TPTZ) complex was reduced to the ferrous form by the antioxidants present in the sample at an acidic pH, to yield an intense blue color that can be monitored by measuring the change in absorbance at 593 nm. The change is directly related to the combined or total reducing power of electron donating non-enzymatic antioxidants present in the reaction mixture. The TAC level was expressed as μM/L.

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Manufacturer protocol

Download the product protocol from Abcam for Rat sE-Selectin ELISA Kit (sCD62E) (ab171334) below.

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